Recombinant Human Signal Transducer CD24/CD24 (C-Fc)

Recombinant Human Signal Transducer CD24/CD24 (C-Fc)

SKU #	PKSH033968
Expression Host	HEK293 Cells

Description

Synonyms	CD24,CD24A,Signal transducer CD24,Small cell lung carcinoma cluster 4 antigen
Species	Human
Expression Host	HEK293 Cells
Sequence	Ser27-Gly 59
Accession	P25063
Calculated Molecular Weight	29.8 kDa
Observed Molecular Weight	33-50 kDa
Tag	C-Fc
Bio-activity	Immobilized Anti-Human CD24 mAb at 2g/ml (100 l/well) can bind Human CD24-Fc: Biotinylated by NHS-biotin prior to testing. The ED50 of Human CD24-Fc is 7.62 ng/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin	< 1.0 EU per g of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80. Reconstituted protein solution can be stored at 4-8 for 2-7 days. Aliquots of reconstituted samples are stable at < -20 for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 m filtered solution of PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background
Signal Transducer CD24 is a heavily and variably glycosylated GPI-linked sialoprotein. Human CD24 is expressed on B lineage cells and granulocytes, on epithelial, neuronal, and muscle cells, and on a range of tumor cells. CD24 expression is regulated during lineage development and with the activation of various cell types. Antibody crosslinking of CD24 enhances the induction of apoptosis in B and T lymphocytes which contributes to negative selection and the induction of immune tolerance. CD24 on antigen presenting cells cooperates with B7 molecules in the costimulation of T cells. CD24 associates in cis with Siglec10 and with the danger-associated molecules HMGB1, HSP70, or HSP90 which are released from necrotic or damaged cells. Formation of these ternary complexes fills a protective role: the resulting Siglec10 signaling inhibits inflammatory responses that are otherwise induced by extracellular DAMPs.